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Design a high-throughput image analysis pipeline using Cell Profiler and ImageJ software.
Immunofluorescence is a widely used tool in the visualization of protein and biomolecules within fixed cultured cells (immunocytochemistry) or preserved and sectioned patient or animal tissue (immunohistochemistry). Fluorescent immunostaining is a reliable confirmation technique used across scientific disciplines both in academia and industry. However, without extremely expensive and proprietary software, analysis and interpretation of this data can be complicated and time-consuming. In this Build Project, you'll wear the hat of a scientist or engineer and build a pipeline capable of delivering high-throughput single cell data from immunofluorescent images. Under the supervision of an experienced Build Fellow, you'll design a segmentation workflow and analyze data. You'll become familiar with industry tools like Cell Profiler and ImageJ. All this will happen in an environment that simulates the operations of a real biotech team.
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