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Perform flow cytometry analysis to characterize cells used in different forms of immunotherapies and effectively visualize data using real-world examples.
Lymphocytes are important white blood cells in the immune system that invade tumors and hold potential for cancer treatment. Understanding their phenotype and functionality through flow cytometry analysis is essential for developing cell therapy drug products and these skills are crucial for building a career in the Immuno-Oncology field. In this Build Project, you will get a solid foundation in flow cytometry to independently analyze basic flow cytometry panels & generate data summaries. You will analyze at least one primary seven or eight-color flow cytometry panel to characterize lymphocytes that can exert anti-tumor activity. You'll also learn to use popular software tools like FlowJo & GraphPad Prism to analyze and visualize data.
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Gauri Kulkarni is an Associate Scientist in the Cell Therapy team at Obsidian Therapeutics and a Molecular Biology Fellow at Open Avenues. She has a Bachelor of Engineering in Instrumentation & Control (with a Biomedical Engineering major) & a MS in Biomedical Engineering. In her current role at Obsidian Therapeutics, Gauri works extensively with isolation, transduction & expansion of Tumor Infiltrating Lymphocytes and has experience performing various functional and characterization assays including multi-parameter flow cytometry, tumor reactivity & cytotoxicity.