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Analyze Flow Cytometry Panels for Cell-Based Immunotherapy
Gauri Kulkarni
Gauri Kulkarni
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Analyze Flow Cytometry Panels for Cell-Based Immunotherapy

Perform flow cytometry analysis to characterize cells used in different forms of immunotherapies and effectively visualize data using real-world examples.

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Apply now
Tuesdays
 at
6:00
P.M.
 ET /
3:00
P.M.
PT
8 weeks, 2-3 hours per week
Intermediate
No experience required
No experience required
Some experience required
Degree and experience required

Description

Lymphocytes are important white blood cells in the immune system that invade tumors and hold potential for cancer treatment. Understanding their phenotype and functionality through flow cytometry analysis is essential for developing cell therapy drug products and these skills are crucial for building a career in the Immuno-Oncology field. In this Build Project, you will get a solid foundation in flow cytometry to independently analyze basic flow cytometry panels & generate data summaries. You will analyze at least one primary seven or eight-color flow cytometry panel to characterize lymphocytes that can exert anti-tumor activity. You'll also learn to use popular software tools like FlowJo & GraphPad Prism to analyze and visualize data.

Session timeline

  • Applications open
    May 27, 2024
  • Application deadline
    June 23, 2024
  • Project start date
    Week of July 8, 2024
    Week of
    July 8, 2024
  • Project end date
    Week of

What you will learn

  • Learn what adoptive cell therapies are and the significance of them in the treatment of cancer
  • Learn what flow cytometry is and how it used in the biotechnology industry
  • Learn how to effectively visualize and communicate your analyzed data for presentation

Project workshops

1
Introductions
2
Cell Therapies
3
Experimental Setup for Flow Cytometry
4
Compensation
5
Practice Analysis
6
GraphPad Prism
7
Real World Examples
8
Presentations

Prerequisites

  • A basic understanding of immunology: you should be able to explain the difference between innate & adaptive immune system and identify the cells of the immune system. There will be a chance to review some of the concepts in the first workshop.
  • Being comfortable using MS PowerPoint, MS Excel and MS Word. Before the project starts, you'll need to have the above-mentioned tools, or their alternatives installed on your computer.

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About the expert

Gauri Kulkarni is an Associate Scientist in the Cell Therapy team at Obsidian Therapeutics and a Molecular Biology Fellow at Open Avenues. She has a Bachelor of Engineering in Instrumentation & Control (with a Biomedical Engineering major) & a MS in Biomedical Engineering. In her current role at Obsidian Therapeutics, Gauri works extensively with isolation, transduction & expansion of Tumor Infiltrating Lymphocytes and has experience performing various functional and characterization assays including multi-parameter flow cytometry, tumor reactivity & cytotoxicity.

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