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Deep Insight with Novel Next Generation Sequencing Assay
Malvenderjit Jagjit Singh
Malvenderjit Jagjit Singh
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Deep Insight with Novel Next Generation Sequencing Assay

Strategize and spearhead the development of an assay aimed at determining the therapeutic potential for curing genetic disorders by leveraging novel generation sequencing approaches

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Apply now
Tuesdays
 at
11:00
A.M.
 ET /
8:00
A.M.
PT
8 weeks, 2-3 hours per week
Intermediate
No experience required
No experience required
Some experience required
Degree and experience required

Description

RNA-seq has been selected as one of the most reliable assays in biotech research for studying gene expression level. In fact, this assay has emerged as a widely used technique for quantifying all gene expression levels. In this Build Project, you’ll imagine you assume the role of a research associate for a well-known company. They have decided to assign you the task of on boarding this assay with the purpose of investigating gene expression levels within the context of assessing the genetic tool your company has developed as part of curing genetic disorders. You will be exposed to common therapeutic approaches being conducted at molecular level and will dive deeper into different next generation sequencing technologies. These experiences represent the daily task of a research associate in the biotech industry and will be a good learning curve as part of your career development.

Session timeline

  • Applications open
    September 5, 2024
  • Application deadline
    September 22, 2024
  • Project start date
    Week of July 8, 2024
    Week of
    October 7, 2024
  • Project end date
    Week of

What you will learn

  • Understand the importance of assay design and its impact as a therapeutic potential in curing genetic disorders.
  • Explore next generation sequencing (NGS) platforms from foundational concepts to fostering critical thinking and bioinformatics approaches.  
  • Learn to successfully perform downstream analysis for RNAseq data with different statistical methods.
Build Projects are 8-week experiences that operate on a rolling basis. Selected participants engage in weekly live workshops with a Build Fellow and 2-15 other students.

Project workshops

1
Introduction to Gene Editing: Course Overview and Technology Development
2
Assay Design Exploration – Part 1
3
Assay Design Exploration – Part 2
4
Assay Design Exploration – Part 3
5
Downstream Analysis – GitHub and Programming Language
6
Downstream Analysis – RNAseq Dataset (Part 1)
7
Downstream Analysis – RNAseq Dataset (Part 1 - Cont'd)
8
Downstream Analysis – RNAseq Dataset (Part 2) & Closing Thoughts

Prerequisites

  • Strong background in molecular biology, including having a good understanding of the central dogma of biology (e.g. transcription and translation).
  • Sufficient level of molecular biology lab experience and techniques including DNA/RNA extraction and PCR amplification to further propagate their understanding in next generation sequencing approaches.
  • Show a strong commitment for continuous learning and professional development by staying abreast with challenges that require problem-solving and technical expertise in next generation sequencing methods.
  • Familiarity in gene editing technologies, like CRISPR-Cas9 technologies, with their underlying principles and applications in therapeutic approaches.

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About the expert
Malvenderjit Jagjit Singh
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Malvenderjit
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Malven, a Molecular Biology Fellow based in Boston, works as a research associate at Tessera, where he focuses on novel assay design and sequencing technologies. Originally from Malaysia, he moved to the United States in 2018 to pursue his undergraduate studies. In his free time, Malven enjoys coding and has a passion for collecting coins and vinyl records.