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Strategize and spearhead the development of an assay aimed at determining the therapeutic potential for curing genetic disorders by leveraging novel generation sequencing approaches
RNA-seq has been selected as one of the most reliable assays in biotech research for studying gene expression level. In fact, this assay has emerged as a widely used technique for quantifying all gene expression levels. In this Build Project, you’ll imagine you assume the role of a research associate for a well-known company. They have decided to assign you the task of on boarding this assay with the purpose of investigating gene expression levels within the context of assessing the genetic tool your company has developed as part of curing genetic disorders. You will be exposed to common therapeutic approaches being conducted at molecular level and will dive deeper into different next generation sequencing technologies. These experiences represent the daily task of a research associate in the biotech industry and will be a good learning curve as part of your career development.
Meet your Build Fellow and get to know the course structure, goals, and requirements, meanwhile we will also be covering the central dogma of molecular biology and its role in gene regulation and explore common genetic disorders like cystic fibrosis and their significance in ongoing research and everyday life.
We will review the evolution of benchmark assays in research and development over recent years, and I’ll provide a technical understanding of DNA mechanisms in replication, repair, and recombination. Additionally, we will discuss how mutations cause genetic disorders thus affecting gene regulation.
In this workshop you'll learn to design effective primers by mastering principles like melting temperature and specificity for PCR, and understand quality control assessments and technical strategies for developing robust assays and overcoming challenges.
In this workshop, we will explore the PacBio’s SMRT and Illumina’s SBS chemistry, and gain insights into various PCR techniques including Illumina’s bridge amplification method.
We will learn to evaluate and summarize RNA-seq findings and get a step-by-step tutorial on further maximizing the use of RStudio/Python for data analysis and using Git as an additional tool for resource.
In this workshop, we will delve into computational models for identifying differentially expressed genes using DESeq2, focusing on negative binomial approaches. We will also learn to develop and customize visualization plots for clearer interpretation of results, aiming for publication-ready standards.
In this workshop, we will delve into computational models for identifying differentially expressed genes using DESeq2, focusing on negative binomial approaches. We will also learn to develop and customize visualization plots for clearer interpretation of results, aiming for publication-ready standards.
We will learn the purpose of enrichment analysis to identify over-represented genes and biological pathways.
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Malven, a Molecular Biology Fellow based in Boston, works as a research associate at Tessera, where he focuses on novel assay design and sequencing technologies. Originally from Malaysia, he moved to the United States in 2018 to pursue his undergraduate studies. In his free time, Malven enjoys coding and has a passion for collecting coins and vinyl records.