Your application

Please complete the following fields to be considered for this project.

Please fill in this required field.
Please fill in this required field.
Please fill in this required field.
Please fill in this required field.
Please fill in this required field.
How much commitment will you have to this project?
Please fill in this required field.
Are you available to dedicate 1-2 hours per week to the Build Project?
Please fill in this required field.
Your application has been 
successfully submitted!
Explore more projects
There was an error submitting your form. Please try again later or contact us.
Oops! Something went wrong while submitting the form.

This project is no longer accepting applications. Subscribe to our newsletter to be notified of new projects!

Get updates
Propose a Method to Optimize the Assessment of Oligonucleotide Purity
Juan Carlos Garcia-Prieto
Juan Carlos Garcia-Prieto
Get updates
Register today
Apply now

Propose a Method to Optimize the Assessment of Oligonucleotide Purity

Research modern liquid chromatography techniques (HPLC) and help improve the method for separation and resolution of oligonucleotides.

Register today
Apply now
 ET /
8 weeks, 2-3 hours per week
No experience required
No experience required
Some experience required
Degree and experience required


Oligonucleotides are synthetically produced biomolecules that have proven to be crucial for gene editing therapies. Biotech companies put a lot of time and effort in optimizing and developing groundbreaking analysis techniques for these molecules; purity assessment being one of the most significant ones. High performance liquid chromatography (HPLC), is one of the tools almost every research team uses to assess and characterize the constituents found in a compound. Knowing this technique becomes a very important skill even for entry-level jobs.

In this Build Project, you'll wear the hat of an Analytical Development Scientist and help optimize a method for purity/integrity assessment of oligonucleotides through RP-IP HPLC. Under the supervision of an experienced Build Fellow, you'll become proficient in navigating scientific literature and building your own conclusions and course of actions in the development of this method. You'll learn the science behind liquid chromatography and how biomolecules such as RNA behaves in such. You will also be able to appreciate role that oligonucleotides play in gene editing therapies; a rising focus for biotech companies. All this will happen in an environment that simulates the operations of a real Analytical Development team.

Session timeline

  • Applications open
    May 27, 2024
  • Application deadline
    June 23, 2024
  • Project start date
    Week of July 8, 2024
    Week of
    July 8, 2024
  • Project end date
    Week of

What you will learn

  • Use research papers to develop and optimize analytical methods.
  • Learn how to apply organic chemistry concenpts to real life scenarios in order to better characterize biomolecules.
  • Learn how to analyze and interpret HPLC data.
  • Understand how HPLC instruments function, including their different modalities and uses.
Build Projects are 8-week experiences that operate on a rolling basis. Selected participants engage in weekly live workshops with a Build Fellow and 2-15 other students.

Project workshops

Gene Editing Therapies
Intro to Oligonucleotides
Intro to HPLC Systems
Using HPLC Techniques for RNA Applications
Role of Ion Pairing Agents in RP-IP HPLC
Method Development


  • Basic knowledge of biochemistry: you should understand the function of DNA in cells and how RNA molecules work in our body.
  • Entry-level knowledge of organic chemistry: you shouldhave basic understanding of  the structure, properties, and reactions of organic compounds such as alcohols, amines and alkyls.
  • Basic/intermediate knowledge of analytical chemistry and instrumental analysis: you should be able to understand how separation techniques such as chromatography are used to analyze compounds and materials.
  • Other prerequisites: before the project starts, you'll need to have google scholarly and Microsoft Word/PowerPoint installed on your computer.

Sign up today

Get access to all of our Build projects, including this one, by creating your Build account!

Register today
Log in

Apply to

Juan Carlos

's project today!

Get started by submitting your application.

Apply now

Stay updated!

Subscribe to our newsletter to be notified when projects reopen!

Please fill in this required field.
By clicking “Subscribe” you agree to our Terms of Services and Privacy Policy.

Thanks for subscribing!

We'll notify you when projects reopen. In the meantime, you can explore our resources and learn more about our Fellows.

Discover our articles
There was an error submitting your form. Please try again later or contact us.
Thank you! Your submission has been received!
Oops! Something went wrong while submitting the form.
About the expert
Juan Carlos Garcia-Prieto
Juan Carlos
's Linkedin

Juan Carlos Garcia-Prieto is a Chemistry Global Talent Fellow at Open Avenues, where he works with students leading projects in Chemistry.

Juan Carlos Garcia-Prieto is an Associate Scientists, Analytical Development at Tome Biosciences where he works on developing and optimizing assays for the quantitation and characterization of biomolecules involved in gene editing therapies.

Juan Carlos Garcia-Prieto has 3 years of experience in Analytical Development.

Prior to joining Tome, Juan Carlos Garcia-Prieto worked at Moderna analyzing and characterizing all the raw materials used to produce vaccines, as well as any other biomolecule used for research purposes.

He holds a degree in Chemistry.

A fun fact about Juan Carlos Garcia-Prieto: He has a 2yo French Bulldog named Bruno.