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Quantify Cytokine Release to Functionally Characterize CAR T-cells Targeting Tumor Antigens 
Sai Valli Srujana Namburi
Sai Valli Srujana Namburi
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Quantify Cytokine Release to Functionally Characterize CAR T-cells Targeting Tumor Antigens 

Learn the biology of CAR T-cells and cytokines with a major focus on quantifying cytokine secretion in response to cancerous tumors.

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Fridays
 at
10:00
A.M.
 ET /
7:00
A.M.
PT
8 weeks, 2-3 hours per week
Intermediate
No experience required
No experience required
Some experience required
Degree and experience required

Description

Cytokines are cell signaling proteins and are released by the CAR-T cells upon engagement with tumor antigens. So, measuring cytokines release is one of the most often used techniques in tumor research. This data is often critical for Investigational New Drug (IND) applications to the FDA. In this Build Project, you'll wear the hat of a Research Associate and quantify a certain set of cytokines to evaluate the functional profile of CAR T-cells before their clinical application. Under the supervision of an experienced Build Fellow, you'll learn how to process the raw data for visual and quantitative analysis using statistical software like MS Excel and GraphPad Prism. You'll also develop skills for drawing meaningful conclusions from the analysis you perform.

Session timeline

  • Applications open
    May 27, 2024
  • Application deadline
    June 23, 2024
  • Project start date
    Week of July 8, 2024
    Week of
    July 8, 2024
  • Project end date
    Week of

What you will learn

  • Knowledge about CAR-T cell therapy and the importance of cytokines
  • Cytokine-release assay design and data analysis
  • Data compilation and scientific presentation
Build Projects are 8-week experiences that operate on a rolling basis. Selected participants engage in weekly live workshops with a Build Fellow and 2-15 other students.

Project workshops

1
Introduction
2
Literature review
3
Journal club
4
Experiment design
5
Data analysis
6
Data interpretation
7
Build final projects
8
Presentations

Prerequisites

  • Basic knowledge of biology: you should understand the concept of cells, immune system and cancer. 
  • Basic knowledge of MS Office: you should be able to navigate through MS Excel and MS PowerPoint. 

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About the expert
Sai Valli Srujana Namburi
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Sai Valli Srujana
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Sai Namburi is a Molecular Biology Build Fellow at Open Avenues, where she works with students leading projects in cell biology. She works for a cell therapy company on the development of CAR T- Cell therapies for cancer. Sai has 6 years of experience in pre-clinical research. She has research experience in both academic research institutions and pharmaceutical industries. Sai holds a Master’s degree in Microbiology and Immunology. A fun fact about Sai is that you can find her biking on CA-1 if she isn't at work.